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Antibodies bound to the blocking peptide no longer bind to the epitope about the target protein. By evaluating the staining from your blocked antibody vs . the antibody on your own, you can see which staining is distinct.
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This information outlines further attainable optimization of mobile and immunoassay parameters to make certain the very best outcomes are...
This tutorial outlines further achievable optimization of mobile and immunoassay parameters to ensure the best possible effects are...
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The antibody is from purified rabbit serum by affinity purification by means of sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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The measurement of protein phosphorylation is a great tool for measuring the modulation of receptor activation by equally antibodies...
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Blocking peptides are peptides that bind especially for the target antibody and block antibody binding. These peptide generally consists of the epitope recognized by the antibody.
Concentrate on Facts Cyclin E belongs to your hugely conserved cyclin household, whose associates are characterized by a extraordinary periodicity in protein abundance from the cell cycle. Cyclins function as regulators of CDK kinases. Distinctive cyclins show distinct expression and degradation designs which contribute into the temporal coordination of every mitotic occasion. Cyclin E forms a fancy with and functions as a regulatory subunit of CDK2, whose action is required for mobile cycle G1/S transition. This protein accumulates at the G1-S period boundary and it is degraded as cells development via S phase.
Overexpression of this gene has been noticed in many tumors, which ends up in chromosome instability, and thus may well contribute to tumorigenesis. This protein was observed to associate with, and become involved in, the phosphorylation of NPAT protein (nuclear protein mapped on the ATM locus), which participates in mobile-cycle regulated histone gene expression and performs a crucial purpose in advertising mobile-cycle development inside the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode distinct isoforms, are already explained. Two extra splice variants ended up reported but in depth nucleotide sequence information and facts isn't nevertheless out there.
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